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          主頁(yè) > 新聞動(dòng)態(tài) > Polysciences你會(huì)喜歡的第二代聚合物轉(zhuǎn)染試劑
          新聞動(dòng)態(tài)
          Polysciences你會(huì)喜歡的第二代聚合物轉(zhuǎn)染試劑

          品牌故事——我的故事里怎能少了你?

          Polyscience,Inc,成立于1961年,起家于化學(xué)但超越化學(xué)領(lǐng)域的美國(guó)制造商,總部位于賓夕法尼亞州沃靈頓,公司業(yè)務(wù)主要涵蓋三大塊:1.實(shí)驗(yàn)用產(chǎn)品:a)成千上萬(wàn)種具品牌特色的化合物和試劑,應(yīng)用于實(shí)驗(yàn)室研究,組織學(xué),電鏡和解剖病理學(xué)以及其他診斷和生命科學(xué)研究;b)種類極其寬廣的單體和聚合體,滿足各方研究領(lǐng)域;c)有機(jī)/無機(jī)化合物,生物可降解材料,磁珠,熒光素,染料和特定抗體,以及常規(guī)蛋白包被顆粒(直徑范圍40nm~10mm)的首要制造工廠;d)頂端多聚化合物的供貨中心,滿足各種高性能工業(yè)領(lǐng)域需求,包括替代能源,汽車電子,國(guó)防&電子,便攜式通信和計(jì)算機(jī),醫(yī)療器械組裝。2. 定制合成;3.cGMP生產(chǎn)服務(wù)。1800523-2575;(215343-6484。

          Brand Story

          Me,PolyscienceIncChemistry beyond the ordinary

          Me,Polyscience,Inc,Headquartered at WarringtonPA,Call vial 1800523-2575

          Me,Polyscience,Inc,Manufacturing hundreds of specialty chemicals and reagents for laboratory research, Histology, Light Microscopy and Anatomic Pathology as well as other clinical and life science applications.

          Me,Polyscience,IncStocking a wide portfolio of monomers and polymers for research applications.

          MePolyscience,Inc,Developed to be the primary source for organic, inorganic, biodegradable, magnetic, fluorescent, dyed and specific antibody and general protein coated particles in diameters spanning the range of 40nm to 10mm.

          Me,Polyscience,IncOffering innovative polymer products to meet the high performance requirements of a variety of industries including alternative energy, automotive electronics, defense & aerospace electronics, portable communications & computing, medical device assembly, and more. 

          ——Written/Edited by V. Shallan

           

          Special Products

          你會(huì)喜歡的第二代聚合物轉(zhuǎn)染試劑

          Polyethylenimine,Linear,Mw 25000 線性化聚乙烯亞胺(PEI 25000

          基本描述:

          線性化聚乙烯亞胺PEI 25,000,一種高電荷陽(yáng)離子聚合物,非常容易結(jié)合于高電荷陰離子基質(zhì)。工業(yè)應(yīng)用上,線性化PEI可改善負(fù)電荷染料的物理外觀,以調(diào)整染料的化學(xué)特性和增強(qiáng)染料與固相基質(zhì)的黏附能力,常用作黏附增強(qiáng)劑,作用同多聚賴氨酸(poly-lysine);科研應(yīng)用上,線性化PEI能與DNA或其他負(fù)電荷生物大分子簡(jiǎn)單結(jié)合,正是這一特性,使得成為一種非常行之有效的載體運(yùn)輸介質(zhì)Vector carrier),即轉(zhuǎn)染試劑。

          化學(xué)性質(zhì):

          CAS NO.

          9002-98-6, 26913-06-4

          Formula

          (CH2CH2NH)n

          Molecular Weight

          25,000

          Melting Point

          73-75º 

          Solubility

          Soluble In: hot water, cold water at low pH, methanol, and ethanol.

          Insoluble In: benzene, ethyl ether and acetone

          Appearance

          White to yellow solid

          訂購(gòu)信息:【*,現(xiàn)貨供應(yīng)】

          品牌

          貨號(hào)

          名稱

          規(guī)格

          *(元)

          Polysciences

          23966-2

          線性聚乙烯亞胺(MW 25,000

          2g

          2684

          另,提供更高轉(zhuǎn)化效率的PEI Max 40,000,自由基形式分子量相當(dāng)于PEI 25000,研究證實(shí)其體外質(zhì)粒DNA轉(zhuǎn)染效率提高達(dá)21倍。

          Polysciences

          24765-2

          線性聚乙烯亞胺鹽酸鹽Max,(MW 40000 (Equivalent to Mw 25,000 in Free Base Form)

          2g

          3184

          使用方法【來源于:CSH protocols,僅作交流學(xué)習(xí)

          1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.

          2. Add 500 mg of PEI to the beaker with stirring.

          3. Add concentrated HCl drop-wise to the solution to pH <2.0.

          4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.

          Approximay 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

          5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

          Approximay 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

          6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.

          7. Filter-sterilize the solution through a 0.22-μm membrane.

          8. Store aliquots of the desired volume at -20°C.

          應(yīng)用文獻(xiàn)【基因轉(zhuǎn)染用途

          Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.

          Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pin (2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors. Journal of Virological Methods. 138(1–2): 85–98.

          Patti A. LongoJennifer M. KavranMin-Sung Kim, and Daniel J. Leahy2013Transient Mammalian Cell Transfection with Polyethylenimine (PEI) . Methods Enzymol. 529: 227–240.

          ——Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】

           

           

          更的PEI聚乙烯亞胺聚合物轉(zhuǎn)染試劑

          Polyethylenimine HCl MAX, Linear, Mw 40,000

          線性化聚乙烯亞胺鹽酸鹽 MAXPEI 40,000

          基本描述:

          Polyethylenimine HCl MAX, Linear, Mw 40,000PEI 40,000)是熱賣產(chǎn)品線性化聚乙烯亞胺PEI25,000的升級(jí)產(chǎn)品,不同之處在于1)*水解(去乙?;?;2PEI25000的鹽酸鹽形式,具更高的水溶特性;3)含更長(zhǎng)連續(xù)性乙烯亞胺(ethyleneimine)片段,其質(zhì)子化氮水平比PEI25,000提高11%以上。本品經(jīng)堿中和后,可轉(zhuǎn)化為線性化自由胺(PEI),此時(shí)分子量正相當(dāng)于25,000。

          Thomas M,et al2005)研究數(shù)據(jù)表明,去乙?;?/span>PEI 40,000Polyethylenimine MAX 40,000)體外質(zhì)粒DNA轉(zhuǎn)染效率提高21倍,小鼠體內(nèi)靶向效率達(dá)到10,000倍,隨之肺部特異療效顯示增強(qiáng)1500倍。

          化學(xué)性質(zhì):

          CAS NO.

          9002-98-6, 26913-06-4

          Molecular Weight

          40,000~22,000 free base

          Solubility

          Soluble In: Cold and room temperature water 

          Insoluble In: Common organic solvents (ethanol, acetone, tetrahydrofuran)

          Appearance

          White to off-white free flowing solid

          訂購(gòu)信息:【*,現(xiàn)貨供應(yīng)】

          品牌

          貨號(hào)

          名稱

          規(guī)格

          *(元)

          Polysciences

          24765-2

          線性聚乙烯亞胺鹽酸鹽Max,(MW 40000 (Equivalent to Mw 25,000 in Free Base Form)

          2g

          3184

          另,提供基本型PEI 25,000。

          Polysciences

          23966-2

          線性聚乙烯亞胺(MW 25000

          2g

          2684

          應(yīng)用文獻(xiàn)【基因轉(zhuǎn)染用途

          Mann JF, McKay PF, Arokiasamy S, Pa RK, Klein K, Shattock RJ. (2013). Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.

          Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lungProc Natl Acad Sci U S A. 102(16):5679-84.

          ——Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】

           

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